黑曲霉E133131菌株生产菊粉酶发酵培养基的优化.rar

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摘要:本实验利用黑曲霉E133131通过深层发酵法进行菊粉酶的生产,并对发酵培养基的碳源、氮源、无机盐等营养物质进行单因素实验研究,优化发酵培养基的配方。

   测定其初始酶活为1.68U/mL,通过与初始酶活的比较确定发酵培养基的最佳单因素为碳源乳糖(11%)、有机氮源牛肉膏(4%)和无机盐硫酸铜(0.9%)。

   最后通过正交试验确定最佳发酵培养基的配方:乳糖(11%)、牛肉膏(4%)、硫酸铜(0.9%)牛蒡汁10%,酵母膏(2%),氯化钠(0.5%),磷酸氢二钾(0.5%),硫酸铵(0.5%),磷酸氢二铵(2%),蒸馏水100mL。在装瓶量80mL/250mL,温度30°C,转速180r/min,的条件下发酵4d,黑曲霉E133131所产菊粉酶的最高酶活为8.91U/mL,比初始酶活提高了430.36%。

关键词 黑曲霉;内切型菊粉酶;酶活;培养基

 

Abstract:This experiment using aspergillus Niger E133131 inulinase production, through the deep fermentation and the fermentation culture medium carbon source, nitrogen source, inorganic salt and other nutrients in the single factor experiments, the optimum fermentation culture medium formula.

   Determine its initial enzyme activity of 1.68 U/mL, comparing with the initial enzyme activity to determine the best single factor as the carbon source of lactose fermentation medium (11%), organic nitrogen source beef paste (4%) and inorganic salt copper sulfate (0.9%).

   Finally through orthogonal experiment the optimum fermentation culture medium formula: lactose (11%), beef extract (4%), and copper sulfate (0.9%), burdock juice 10%, yeast extract (2%), sodium chloride (0.5%), potassium hydrogen phosphate (0.5%), ammonium sulfate (0.5%), diammonium phosphate (2%) and 100 ml of distilled water. In bottle of 80 mL / 250 mL, 30 ° C temperature, rotating speed of 180 r/min, the conditions of fermentation, 4 d, aspergillus Niger E133131 inulinase production by the highest enzyme activity of 8.91 U/mL, a 430.36% increase over the initial enzyme activity.

Keywords  aspergillus niger  cut type inulinase  enzyme activity  medium

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